DNA sequencing technique and applications

DNA sequencing

What is DNA sequencing? ”DNA sequencing is a technique of biotechnology to determine the genome sequence with precise nucleotide order within it.” Determination of precise sequence and order of DNA bases: adenine, guanine, cytosine and thymine is performed. The purpose is to analyze and compare the genetic makeup of individuals. Moreover, it has significance in …

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Nutritional biochemistry and metabolism

Nutritional biochemistry

The branch of science that interprets or explains the relationship of food with the functioning of living system is nutritional biochemistry. Nutrition refers to all kind of nourishment that is helpful for sustainability of life. The study of nutrient requirement and the diet that provides these nutrients is also termed as nutrition. So, the nutrition …

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bacterial transformation and selection of cells

Bacterial transformation and selection

Transformation is a basic technique of biotechnology to develop transformed cells (bacterial, plant or animal cell) containing a foreign DNA fragment in their genome. The foreign DNA incorporates in cellular genome in such a way that genes of foreign DNA express themselves. Therefore, the organism or cell is termed as transformed cell. Consequently, the transformed …

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Various types of Recombinant antibodies

Recombinant antibodies

The B cells of adapted immune system produces antibodies or immunoglobin molecules in response to foreign antigens. The emergence of recombinant DNA technology in 1970’s has led to the production of ‘engineered or recombinant’ antibodies (rAbs). Initially researchers tried their best for production of humanized antibodies and fragmentation of these Abs and it resulted in …

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Genetic mutations and types

Genetic mutations

Genetic mutations are spontaneous changes occurring in the genetic material, (DNA) of an individual. Mutations can be passed to the next generations or may not, depending upon the type of cell in which they occur. They can be harmful, beneficial or even neutral. Usually these are caused naturally but they can be caused by harmful …

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Gene therapy for therapeutic purposes

Gene therapy

Gene therapy is technique of recombinant DNA technology which involves the replacement of faulty or mutated gene with normal one, mainly for the treatment purpose. DNA, as we know is the genetic and hereditary material of cell that determines and control the cellular functioning. Any mutation or sudden change in gene structure leads to loss …

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Numbness of feet in Diabetic neuropathy

Diabetic neuropathy

Diabetes can be harmful for nerves and may cause nerve damage. This condition is known as peripheral neuropathy or diabetic neuropathy. Nerves, on the other hand, are specialized form of tissues in brain that conduct messages from brain to other parts of body. Nerves play vital role in nervous coordination which eventually controls and regulate …

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Mechanism of Enzyme catalysis

Enzyme catalysis

The rate of chemical reaction is enhanced by the enzymatic action on substrate molecule, a process named as enzyme catalysis. Enzyme catalysis is vital for certain biochemical reactions in cells as without the enzymatic actions the original rate of reaction is too low. Enzymes reduced the activation energy of reaction so that more molecules are …

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Receptors for immune system

Receptors of immune system

Introduction Receptors for innate immune system are specialized proteins that recognize foreign particles and the pathogenic bacteria. Consequently, the innate immune system generate specific responses in response to these receptor proteins, either itself or by activating the cells of adaptive immune system. The innate immune system of body comprises of certain type of cells and …

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Enzyme specificity and mechanism of enzyme action

Enzyme specificity

Enzyme specificity describes the tendency of an enzyme to catalyze a specify biochemical reaction by binding to specific substrate. Enzymes are specific for the type of reaction that they catalyze. For example, the digestive enzymes break down the macromolecular food particle into small digestible particles. Trypsin enzyme catalyzes the breakdown of proteins. Amylase enzyme catalyzes …

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Immunoglobulins

Immunoglobulins (Ig) or antibodies (Ab) are glycoprotein molecules produced by the plasma cells in response to immunogen that may be any foreign particle. These are called as immunoglobulins as they consist of globular proteins in their structure. Basic structure of immunoglobulin Each immunoglobulin molecule consists of four polypeptide chains in which two are light and …

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Sjogren's syndrome

Sjogren’s syndrome

Sjogren’s syndrome is an auto immune disorder and mainly affects the moisture-producing cells and glands of body. The disease is characterized by dry mouth, eyes, and skin and vaginal dryness. Numbness of muscles, gastric ulcer and joint pain and lymphoma are other symptoms. This disease affects the mucous membrane and moisture-secreting of eyes and mouth …

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Cancerous white blood cells in leukemia

Leukemia

Leukemia is type of blood cancer which affects the blood cells usually the white blood cells and bone marrow. White blood cells are integral part of immune system and the bone marrow is where blood cells are made. People with leukemia have large number of abnormal white blood cells. These abnormal cells develop rapidly and …

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History of animal cell culture

Introduction “The process by which the cells are grown outside the body of organism, in artificial environment as in laboratory is termed as cell culturing and the cells are termed as cell culture.” In laboratory for experimental and research purpose, the researcher grow cells under certain specific conditions. These are temperature, pH, concentration of certain …

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Lung cancer treatment options

Lung cancer (part 2)

Lung cancer treatment or therapy depends on the stage of cancer and varies from person to person. Sometimes doctors perform combined therapies for curing cancer. The theray may have two purposes: Curative: These are for the purpose of cure and treatment of lung cancer. Palliative: if the cancer become malignant as in stage 4, treatment …

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